Gene Expression Services
Request Forms:
Digital Droplet PCR Request
Real-Time PCR Registration
Real-Time PCR Request
Equipment Used:
BioRad Qx200 Droplet Digital PCR (ddPCR) System
QuantStudio 7 Pro Real-Time PCR System
Digital Droplet PCR
Droplet Digital PCR technology is a digital PCR method utilizing a water-oil emulsion droplet system. Droplets are formed in a water-oil emulsion to form the partitions that separate the template DNA molecules.
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SERVICE |
INTERNAL |
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One column (8 reactions) |
$45 |
RT-PCR: QuantStudio 7 Pro
This system makes it simple and easy to get high-quality real-time PCR results. This 96/384 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.
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SERVICE |
INTERNAL |
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Full service |
$2.50 per reaction (clients provide RNA samples and primers) |
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Self-service |
$50 per year for registration and $5 per plate run |
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High-resolution melting curve |
$3 per reaction (clients provide RNA samples and primers) |
FAQs
How much DNA do I need?
This can vary depending on application and scope of the project. Please reach out to us for a consultation.
What dye do you use for RT-PCR?
We use SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix which contains SYBR Green I dye for our RT-PCR reactions.
How does digital droplet PCR work, and what makes it different from RT-PCR?
In RT-PCR, a single sample is measured over time, but in Droplet Digital PCR, the sample is partitioned into 20,000 nanoliter-sized droplets. ddPCR provides better precision (±10%) and is capable of detecting small fold-changes in gene expression (1.2x-fold).
Should I use RT-PCR or ddPCR?
ddPCR is preferred for quantifying rare DNA targets, or in cases where a small difference between samples must be detected. Otherwise, traditional RT-qPCR can be used to save costs.
Can you provide primer design?
We can provide guidance, but do not design primers. Please reach out to us for a consultation.