Quality Assurance
Accurate and fast
Sequencing
Competitive pricing for cutting-edge instruments
Analysis
Publication-ready figures
Next Generation Sequencing Fees
The Illumina NextSeq 1000 is an NGS sequencing system designed to miniaturize sample and reagent volumes and increase read output. The NextSeq 1000 is capable of many sequencing applications, including targeted metagenomics, whole genome sequencing, transcriptomics, immune repertoire profiling, sequencing of targeted panels, exomes and large panels, circulating tumor DNA, single-cell sequencing, and spatial profiling.
Contact the facility to discuss services and applications.
| SERVICE | INTERNAL | ACADEMIA |
|---|---|---|
| Amplicon library preparation | $50 | $65 |
| 16S Profiling | $50 | $65 |
| Illumina DNA library preparation | $130 | $145 |
| SequelPrep normalization | $155 | $200 |
| Kapa qPCR validation | $50 | $50 |
| NovaSeq PE150bp (50M reads) | $155 | Contact Facility |
| RNA-Seq Library Prep (PolyA capture) | $100 | Contact Facility |
| RNA-Seq Library Prep (Ribodepletion) | $150 | Contact Facility |
| NextSeq cartridges | ||
| 300 cycle (1 x 150) P1 | Contact facility | Contact facility |
| 600 cycle (2 x 300) P1 25M Reads | $1425 per 25M reads | $1600 per 25M reads |
Sanger Sequencing Fees
Sample submission considerations.
The facility provides a number of standard sequencing primers for common plasmid vectors.
Custom primers should be provided at a concentration of 5 uM (=5 pmol/uL).
Request Forms:
Individual Sample Submissions
Sanger Sequencing Request - Use this form to submit DNA templates (ie. PCR amplicons, Plasmids, etc.) for sequencing
DIY Sequencing Request - Use this for to submit completed flourescent Sanger products (ie. BigDye reactions) for sequencing
For full and half-plate submissions please contact the facility
Equipment Used: Applied Biosystems 3730 DNA analyzer
| SERVICE | INTERNAL | ACADEMIA | COMMERCIAL |
|---|---|---|---|
| Single reaction | $9 | $9 | $16 |
| 96 Reactions (Full Plate) | $500 | $600 | $750 |
| DIY Cycle Sequencing - single reaction | $5 | $6 | $8 |
| DIY Cycle Sequencing - up to 48 reactions | $75 | $100 | $150 |
| DIY Cycle Sequencing - 96-well plate | $150 | $200 | $300 |
| Fragment Analysis - up to 48 reactions | $50 | $75 | $125 |
| Fragment Analysis - 96-well plate | $100 | $150 | $250 |
Note:
DIY Cycle Sequencing is performed by clients with using a Sanger Sequencing Mix (ie. BigDye Terminator V3.1 Mix).
Sanger mix can be purchased separately from the facility using the AAC Consumable Order Form.
Gene Expression Services
Request Forms:
Digital Droplet PCR Request
Real-Time PCR Registration
Real-Time PCR Request
Equipment Used:
BioRad Qx200 Droplet Digital PCR (ddPCR) System
StepOnePlusTM Real-Time PCR System
Digital Droplet PCR
Droplet Digital PCR technology is a digital PCR method utilizing a water-oil emulsion droplet system. Droplets are formed in a water-oil emulsion to form the partitions that separate the template DNA molecules.
| SERVICE | INTERNAL |
|---|---|
| One column (8 reactions) | $45 |
RT-PCR: StepOnePlus™
This system makes simple and easy to get high-quality real-time PCR results. This 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.
| SERVICE | INTERNAL |
|---|---|
| Full service | $2.50 per reaction (clients provide RNA samples and primers) |
| Self-service | $50 per year for registration and $5 per plate run |
| High-resolution melting curve | $3 per reaction (clients provide RNA samples and primers) |
DNA/RNA Quantification
Qubit
Fluorometric concentration assays are generally more accurate than spectrophotometric/UV-Vis absorbance (ie. Nandrop) measurements because they are less affected by common contaminants (ie. Proteins, RNA, or DNA).
This method is recommended NGS library assessments and for any application that requires higher accuracy in concentration estimates than can be acheived with Nanodrop.
Request Form:
Qubit Request
Equipment Used: Qubit Flex Fluorometer, Qubit® 2.0 Fluorometer
| SERVICE | INTERNAL |
|---|---|
| Qubit analysis | $1.50 per sample, plus $3 for standards |
Nanodrop
The NanoDrop® ND-8000 can measure the concentration and quality of RNA, DNA, protein or labelled probes for microarray analysis. It is designed for the use of 1 ul samples. Cuvettes are not required. NanoDrop® ND-8000 can measure 8 samples simultaneously.
Subscription Form: https://www.uoguelph.ca/aac/facilities/genomics/documents/aac-geldoc-and-or-nanodrop-subscription
| SERVICE | INTERNAL |
|---|---|
| Nanodrop | $50 per year |
Agilent TapeStation 4150
The TapeStation system allows for automated electrophoresis of most DNA and RNA samples. Typical applications include quality control (QC) of NGS libraries and RNA extractions (ie. RIN). This method excels in the determination of fragment length distribution of nucleic acid samples, but is not typically relied on for accurate quantification. The TapeStation can be used in place of the BioAnalyzer for most applications, but cannot currently be used for small/micro RNA analysis.
Service includes Standard and High Sensitivity assays for RNA, DNA >1kb, and DNA >5kb, as well as a Standard gDNA assay.
Request Form: AAC TapeStation Request Form
Equipment Used: Agilent TapeStation 4150
| Assay | Set-up | Per Sample |
|---|---|---|
| RNA | $10.50 | $5.50 |
| HS RNA | $12.00 | $6.25 |
| D1000 | $8.00 | $5.50 |
| HS D1000 | $11.00 | $7.75 |
| D5000 | $12.75 | $5.25 |
| HS D5000 | $17.25 | $7.75 |
| gDNA | $8.75 | $8.75 |
Set-up fee is a flat fee charged per submission in addition to the per sample charge.
Set-up fee covers DNA/RNA ladders, technician time, and instrument overhead. Contact facility for more information about ladders and when to use them.
Bioinformatics
We provide comprehensive bioinformatics services, including quality control, trimming, transcriptomics, metagenomics, assembly, 16s and other amplicon analysis, and variant calling.
We are capable of providing custom analyses not listed here. For example, single cell sequencing, proteomics analysis, multifactorial statistical analysis, or genome/transcriptome annotation.
Fees
| Service | Internal | Academia | Commercial |
|---|---|---|---|
| Standard Pipelines | |||
| Quality Control and Trimming | $50 | $65 | $80 |
| Transcriptomics (Differential Expression) | $300 | $390 | $480 |
| Metagenomics | $200 | $260 | $320 |
| Genome Assembly (Bacterial) | $200 | $260 | $320 |
| Variant Calling | $300 | $390 | $480 |
| 16s/18s/23s/ITS | $400 | $520 | $640 |
| Custom Analysis | |||
| Hourly Labour Rate | $80 | $100 | $120 |
About Us
The Genomics Facility is one of five highly specialized laboratories in the University of Guelph's Advanced Analysis Centre. The AAC is located in the multidisciplinary Summerlee Science Complex which provides a range of capabilities for research and advanced education at the interface of the physical and biological sciences.
The Genomics Facility is designated as an Ontario Genomics Platform Affiliate, OGPA, service facility and operates as a core facility offering molecular biology technology support services and training in DNA sequencing, genotyping, and gene expression analyses to the University of Guelph as well as other academic, government and private sector institutions.
Contact Us
Room 1401, Summerlee Science Complex
University of Guelph
488 Gordon St. Guelph, ON N1G 2W1
519-824-4120 ext: 58357
genomics@uoguelph.ca
Facility Hours:
Monday to Friday
9:00 am - 4:30 pm