Protein ID info

Protein Identification Workflow

$Workflow for protein identification.Proteins are extracted and can be fractionaled,enriched or separated on a gel followed by digestion with protease into small peptides between 8 and 30 aminoacids. These peptides can first be enriched or fractionaled followed by a clean-up and then separated using chromatography and detected by mass spectrometry and fragmented. The data is then analyzed using Peaks 10.0 software and compared to protein databases which leads to protein identification.$

If you plan to submit any samples for protein identification, it is important that you read the information in this section, to save time and money by avoiding common mistakes.

Sample preparation guidelines for protein ID [1]
Co-IP: [2]Co-IP [3], MS compatible Flag tag Co-IP protocol [4], endogenous Co-IP [5],
Protocol for solution digestion [6]
Protocol for in-gel digestion [7]